The FOXP3 antibody is a 431 amino acid protein and a member of the forkhead/winged-helix family of transcriptional regulators. It is highly conserved across all mammals. It is essential for normal immune homeostasis and is constitutively and stably expressed at high levels in CD25 + CD4 positive regulatory T-cells, as well as at low levels in CD4 positive/CD25 negative cells. It is entirely absent in CD4 negative/CD8 positive T-cells. It could be a master regulatory gene and a better marker for regulatory T-cells than others.
More Information
The FOXP3 antibody is designed for research purposes only. It comes in a polyclonal antibody with no known clone and a monoclonal antibody with a clone called SP97. The immunogen is the synthetic peptide that is derived from the C-terminus of the human FoxP3 protein. The isotype is the Rabbit IgG and has an undetermined epitope. The molecular weight is 50kDa.
Procedure/Application
Whether you choose polyclonal or monoclonal, you will be using the Immunohistochemistry application. You should only use paraffin-embedded or Formalin-fixed tissues. Slides should be deparaffinized using xylene, a xylene alternative or graded alcohols.
When choosing the concentrated version, you will need to dilute the antibody using a ratio of one to 200. You can also find a pre-diluted formulation which may be suitable for your needs.
To retrieve the antigen, you will need to boil the tissue section in a 1mM EDTA with a pH of 7.0 for ten minutes, and then allow it to cool to room temperature for 20 minutes. The incubation period is 30 minutes while at room temperature. You should rinse the slides with a PBS/0.05% Tween between steps.
The positive control is the tonsil with cellular localization occurring in the nucleus.
The FOXP3 antibody is the perfect option for many research needs. Visit Spring Bioscience now to learn more.